Voprosy meditsinskoi khimii (ISSN 0042-8809)

Microbiological determination of DNA levels in blood leukocytes

Blokh K.O., Paskevich I.F.
PubMed Id: 3082071
Year: 1986 vol: 32  issue:1  pages: 134-136
Abstract: A procedure is developed for estimation of DNA microquantities in biological samples, which involved acid hydrolysis of DNA and the following microbiological estimation of its concentration. A sensitivity of the procedure was as little as 0.25 micrograms DNA per ml. At the DNA concentration 0.25-10 micrograms/ml growth of the indicator strain of bacteria exhibited the linear shape. As shown by means of the procedure developed one rat leukocyte contained 6.3 +/- 0.6 X 10(-12) g of DNA.
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Reference: Blokh K.O., Paskevich I.F., Microbiological determination of DNA levels in blood leukocytes, Voprosy meditsinskoi khimii, 1986, vol: 32(1), 134-136.