Voprosy meditsinskoi khimii (ISSN 0042-8809)

Acid glycosidases in leukocytes of patients with chronic and acute myeloid leukemias

   
Minakova A.L., Preobrazhenskaia M.E., Khoroshko N.D.
PubMed Id: 3010566
Year: 1986 vol: 32  issue:2  pages: 114-118
Abstract: Activities of five acid glycosidases were examined in leukocytes of 30 healthy persons, 17 patients with chronic myeloid leukemia (CML) and 7 patients with acute myeloid leukemia (AML). In leukocytes of the patients with CML activities of alpha-D-mannosidase, N-acetyl-beta-D-hexosaminidase and beta-D-glucurodase were significantly higher (p less than 0.01) and those of alpha-D-galactosidase and alpha-D-glucosidase were somewhat higher (p less than 0.05) as compared with control leukocytes. Activities of all five glycosidases in leukocytes of patients with AML were within the normal limits. Glycosidase activities were also examined in leukocytes of three groups of patients with CML at different stages of the disease: chronic, progressive and blast crisis. All the enrymes exhibited the highest activity in the first group of patients; the activities of these enxymes in the second group were lower and those in the third group were close to normal. When CML leukocytes were fractionated using the Ficoll-verografin method, the activities of the enzymes in the interface fraction (unmatured cells) were higher than those in the bottom fraction (matured granulyocytes). These data suggest that the increased glycosidase activity in CML cells is due to the presence of the population of unmatured granulocytes which are distinct from blast cells.
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Reference: Minakova A.L., Preobrazhenskaia M.E., Khoroshko N.D., Acid glycosidases in leukocytes of patients with chronic and acute myeloid leukemias, Voprosy meditsinskoi khimii, 1986, vol: 32(2), 114-118.
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