Voprosy meditsinskoi khimii (ISSN 0042-8809)

The use of highly purified staphylococcal alpha-toxin in the immunochemical purification of antibodies

   
Baschenko I.A., Khavkin I.A.
PubMed Id: 3532530
Year: 1986 vol: 32  issue:4  pages: 27-32
Abstract: A simple procedure is described for isolation of highly purified staphylococcal alpha-toxin (SAT) by means of sorption on macroporous glass beads and gel filtration. Various conditions were tested of affinity immunosorption of antibodies and SAT. Highly-porous polyacrylamide gel activated by glutaraldehyde was found as a readily available and selective immunosorbent. The immobilized antigen was stabilized using reduction of azomethyne bonds. Specific activity of antibodies was elevated as the SAT purification degree increased. Correlation between antihemolytic and antilethal activities was unaltered during the immunochemical purification of antibodies.
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Reference: Baschenko I.A., Khavkin I.A., The use of highly purified staphylococcal alpha-toxin in the immunochemical purification of antibodies, Voprosy meditsinskoi khimii, 1986, vol: 32(4), 27-32.
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