Voprosy meditsinskoi khimii (ISSN 0042-8809)

Isolation of membrane antigens of mammary gland epithelial cells from membranes of human milk fat globules

   
Iakubovskaia R.I., Korosteleva M.D., Aleksander S.K., Avdeev G.I.
PubMed Id: 3776115
Year: 1986 vol: 32  issue:5  pages: 36-41
Abstract: Seven antigens, belonging to various structures of bilayer: ArI (lactoferrin) and Ar5 (alpha-lactalbumin) - adsorbed proteins; Ar2 and Ar14 - peripheric components; Ar3, Ar4 and Ar13 - integral structures, were found after treatment of fatty globule membranes from human milk using the agents with improved extracting activity (isotonic solution, 1 M MgCl2, 1% Triton X-100, combined effect of 1% Triton X-100 and sonication). The procedure developed enabled to separate these antigens and to isolate them from the main milk secretory proteins lactoferrin and alpha-lactalbumin. Towards highly purified preparations of the membrane antigens 3 and 4 monospecific antisera were raised, which allowed to identify these components among tissue-specific antigens of mammary gland capable to be maintained in malignization of the tissue. Ar4 was also found in tumoral tissues of ovary, lung and stomach.
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Reference: Iakubovskaia R.I., Korosteleva M.D., Aleksander S.K., Avdeev G.I., Isolation of membrane antigens of mammary gland epithelial cells from membranes of human milk fat globules, Voprosy meditsinskoi khimii, 1986, vol: 32(5), 36-41.
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