Voprosy meditsinskoi khimii (ISSN 0042-8809)

Methods of determining lipid peroxidation products in the serum using a thiobarbituric acid test

   
Gavrilov V.B., Gavrilova A.R., Mazhul' L.M.
PubMed Id: 2437702
Year: 1987 vol: 33  issue:1  pages: 118-122
Abstract: Main principles for carrying out of the thiobarbituric acid (TBA)-test of blood serum were characterized. These principles involved incubation of the sample at pH 1.3-1.6, extraction of the reaction products with butanol and spectrofluorimetric or spectrophotometric estimation. Content of TBA-active products in blood serum of healthy donors constituted 3.86 +/- 0.66 nmol malonic dialdehyde per ml of blood serum as shown in fluorimetric estimation by means of the procedure developed.
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Reference: Gavrilov V.B., Gavrilova A.R., Mazhul' L.M., Methods of determining lipid peroxidation products in the serum using a thiobarbituric acid test, Voprosy meditsinskoi khimii, 1987, vol: 33(1), 118-122.
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