Voprosy meditsinskoi khimii (ISSN 0042-8809)

Determination of L-amino acid oxidase activity

   
Berezov T.T., Lukasheva E.V., Smirnova I.P.
PubMed Id: 2883770
Year: 1987 vol: 33  issue:1  pages: 127-132
Abstract: Activity of L-amino acid oxidases was studied using several procedures. Optimal concentrations of L-lysine-alpha-oxidase, suitable for each procedure, were established involving highly purified preparations of the enzyme from Trichoderma sp. Estimation of the enzymatic activity carried out by means of calculation of the reduced cofactor accumulated led to two-fold exceeding of the results. The most sensitive procedure was based on evaluation of ammonium content in the reaction catalyzed by glutamate dehydrogenase and the procedure where peroxidase and o-dianizidine were used.
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Reference: Berezov T.T., Lukasheva E.V., Smirnova I.P., Determination of L-amino acid oxidase activity, Voprosy meditsinskoi khimii, 1987, vol: 33(1), 127-132.
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