Voprosy meditsinskoi khimii (ISSN 0042-8809)

Interaction of glucocorticoids with receptors of rat liver cells during the growth of Walker carcinosarcoma

   
Krauialis K.I., Diashrius A.P., Ulinskaĭte A.P.
PubMed Id: 3577055
Year: 1987 vol: 33  issue:1  pages: 33-40
Abstract: Specific glucocorticoid hormone receptors with Kass = 1.23 X 10(8) M-1 and the content of binding sites 5.59 X 10(-13) moles per mg of protein were found in liver cytosol of intact rats. During growth of Walker carcinosarcoma concentration of binding sites decreased approximately by 40%, whereas the affinity of receptors to the hormone did not mainly altered. At the same time, a considerable decrease was noticed in the content of free binding sites not bound with endogenous corticosteroids. After thermoactivation about 50% of 3H-dexamethasone receptor complexes acquired the ability to bind DNA, while in the same experimental conditions only about 20% of the complexes were activated in cytosol of tumor-bearing rats. With growing of the tumor thermal inactivation of the hormone-receptor complexes was not practically altered both in presence and in absence of molybdate ions. In a solution with low ion strength at 25 degrees a half-life dissociation of 3H-dexamethasone-receptor complexes was equal to 0.042 and 0.084 min-1, respectively, in cytosols of healthy and tumor-bearing rats.
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Reference: Krauialis K.I., Diashrius A.P., Ulinskaĭte A.P., Interaction of glucocorticoids with receptors of rat liver cells during the growth of Walker carcinosarcoma, Voprosy meditsinskoi khimii, 1987, vol: 33(1), 33-40.
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