Voprosy meditsinskoi khimii (ISSN 0042-8809)

Purification of human alpha-1-protease inhibitor and production of antisera for its immunochemical determination

   
Basis V.Yu., Bumialis V.A., Kotova T.S.
PubMed Id: 3495068
Year: 1987 vol: 33  issue:1  pages: 54-59
Abstract: Human homogeneous alpha-I-inhibitor of proteases was isolated and purified by means of chromatography on DEAE cellulose, hydroxyapatite and concanavalin Sepharose 4B. After immunization of rabbits specific monovalent antiserum towards the inhibitor was produced. The antiserum was used in diagnosis of hereditary deficiency of alpha-I-inhibitor of proteases and for estimation of its content in newborns.
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Reference: Basis V.Yu., Bumialis V.A., Kotova T.S., Purification of human alpha-1-protease inhibitor and production of antisera for its immunochemical determination, Voprosy meditsinskoi khimii, 1987, vol: 33(1), 54-59.
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