Voprosy meditsinskoi khimii (ISSN 0042-8809)

A method of isolating plasma membranes from adipose tissue of rats without preliminary treatment with collagenase

   
Egutkin G.G.
PubMed Id: 2833029
Year: 1987 vol: 33  issue:6  pages: 132-135
Abstract: A rapid procedure has been described for preparation of a relatively pure fraction of plasmatic membranes from adipose tissue without treatment with collagenase. Gentle homogenization of rat fatty tissue in a buffered sucrose solution yielded membrane fractions that could be separated from the bulk of contaminating mitochondria and microsomes by a series of differential and isopycnic centrifugations. The preparation obtained was enriched 12-fold with 5'-nucleotidase as compared with the initial homogenate and contained minimal contaminations with mitochondria or elements of endoplasmic reticulum.
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Reference: Egutkin G.G., A method of isolating plasma membranes from adipose tissue of rats without preliminary treatment with collagenase, Voprosy meditsinskoi khimii, 1987, vol: 33(6), 132-135.
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