Voprosy meditsinskoi khimii (ISSN 0042-8809)

The use of a radioimmunologic method of analysis of 11-deoxycortisol for the evaluation of the function of the pituitary-adrenal cortex system during the metopirone test

   
Goncharov N.P., Katsiya G.V., Butnev V.Yu., Vorontsov V.I., Khagundiukova L.B., Veseman R.
PubMed Id: 3369121
Year: 1988 vol: 34  issue:1  pages: 130-123
Abstract: A radioimmunoassay of II-deoxycortisol in human and animal blood plasma was developed. The antiserum used in the method was prepared against a conjugate of II-deoxycortisol-3(0-carboxymethyloxime) and bovine serum albumin. Specificity of the antiserum was determined in cross reactions using 32 steroids. High specificity of the antiserum allowed to determine II-deoxycortisol without preliminary chromatographic isolation. Sensitivity of the method enabled to detect 25 pg of II-deoxycortisol. The intra- and interassay variations were less than 10% and 15%, respectively. The method was used for evaluation of hypophysis-adrenal cortex system functions under the conditions of metopirone test.
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Reference: Goncharov N.P., Katsiya G.V., Butnev V.Yu., Vorontsov V.I., Khagundiukova L.B., Veseman R., The use of a radioimmunologic method of analysis of 11-deoxycortisol for the evaluation of the function of the pituitary-adrenal cortex system during the metopirone test, Voprosy meditsinskoi khimii, 1988, vol: 34(1), 130-123.
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