Voprosy meditsinskoi khimii (ISSN 0042-8809)

Reduction of nitroblue tetrazolium by thiols in the presence of vitamin K

   
Gerasimov A.M., Zakharov A.S.
PubMed Id: 3400179
Year: 1988 vol: 34  issue:2  pages: 102-105
Abstract: Thiol-containing proteins and low molecular sulfhydryl-containing compounds reduced nitroblue tetrazolium (NBT) in presence of vitamin K3(2-methyl-1,4-naphthoquinone). Cysteine and dithiothreitol (but not glutathione) reduced NBT also in absence of the quinone. Reduction of NBT was accelerated in the weakly alkaline medium and occurred both via O2- -dependent and O2- -independent mechanism. In presence of quinones the thiols appear to affect the manifestation of oxidoreductase reactions estimated by means of NBT-test.
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Reference: Gerasimov A.M., Zakharov A.S., Reduction of nitroblue tetrazolium by thiols in the presence of vitamin K, Voprosy meditsinskoi khimii, 1988, vol: 34(2), 102-105.
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