Voprosy meditsinskoi khimii (ISSN 0042-8809)

Ortho-dianizidine micromethod of determining L-phenylalanine-alpha-oxidase activity

   
Smirnova I.P., Berezov T.T.
PubMed Id: 3400184
Year: 1988 vol: 34  issue:2  pages: 129-131
Abstract: A micromethod is developed for estimation of 1-phenylalanine-alpha-oxidase activity from Trichoderma sp. The method is based on interaction of H2O2 formed in the reaction with o-dianisidine.HCl. Optimal conditions were adopted for estimation of the new enzyme activity from Trichoderma sp. The enzyme had Km = 2.5.10(-3) M and Vmax = 0.42 U/mg at 20 mM concentration of phenylalanine in the incubation mixture; the highest enzymatic activity was estimated in citrate buffer, pH 5.0. The method is simple, precise and reproducible.
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Reference: Smirnova I.P., Berezov T.T., Ortho-dianizidine micromethod of determining L-phenylalanine-alpha-oxidase activity, Voprosy meditsinskoi khimii, 1988, vol: 34(2), 129-131.
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