Voprosy meditsinskoi khimii (ISSN 0042-8809)

Radioimmunological analysis of 17 beta-estradiol and the effect of physical exertion on its levels in human blood

   
Morozov V.I., Priiatkin S.A., Isakova T.I., Lushchitskaia I.M., Rogozkin V.A.
PubMed Id: 3420801
Year: 1988 vol: 34  issue:3  pages: 136-139
Abstract: Radioimmunoassay (RIA) for estradiol-17 beta (E2) in blood serum was developed. Antiserum against E2 was raised by means of rabbit immunization with 6-(O-carboxymethyl)oxime E2-BSA. Cross reactions of antiserum were studied with 22 natural and synthetic steroids. Antiserum was used at final dilution 1: 75,000. The sensitivity of RIA was 3 pg/tube, the detection efficiency was 83-120%, when 25-800 pg/ml E2 was added. The variation coefficients were 7.8% and 13% (intraassay and interassay, respectively). As shown by the RIA the intensive physical exercises resulted in the increase of E2 concentration by 26% in male athletes blood serum.
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Reference: Morozov V.I., Priiatkin S.A., Isakova T.I., Lushchitskaia I.M., Rogozkin V.A., Radioimmunological analysis of 17 beta-estradiol and the effect of physical exertion on its levels in human blood, Voprosy meditsinskoi khimii, 1988, vol: 34(3), 136-139.
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