Voprosy meditsinskoi khimii (ISSN 0042-8809)

The use of monoclonal antibodies for the quantitative determination of myoglobin by the solid phase immunoenzyme "sandwich" assay

   
Iuronen E.I., Viiksmaa M.G., Mikel'saar A.V.
PubMed Id: 3057738
Year: 1988 vol: 34  issue:4  pages: 132-136
Abstract: A procedure of solid phase single step immunoenzyme assay of the "sandwich" type involving monoclonal antibodies was developed to estimate myoglobin in blood serum of patients, 30 stable hybridomic clones. producing antibodies to not less than six various epitopes of myoglobin, were obtained. Content of myoglobin in blood serum was studied using antibodies of the IgG type to two various epitopes of myoglobin as well as of their conjugates with horseradish peroxidase. The procedure developed enabled to estimate myoglobin in blood serum at concentrations 2-1,600 ng/ml and to analyze 120 preparations within 3 hrs.
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Reference: Iuronen E.I., Viiksmaa M.G., Mikel'saar A.V., The use of monoclonal antibodies for the quantitative determination of myoglobin by the solid phase immunoenzyme "sandwich" assay, Voprosy meditsinskoi khimii, 1988, vol: 34(4), 132-136.
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