Voprosy meditsinskoi khimii (ISSN 0042-8809)

Radioreceptor method of determination of opioid peptides in the rat brain

   
Davletov E.G., Kurochkin I.N., Zaĭtsev S.V.
PubMed Id: 2848361
Year: 1988 vol: 34  issue:4  pages: 136-138
Abstract: A procedure is described for quantitative radioreceptor estimation of opioid peptides in rat brain using lyophilized membrane preparations. As opiate receptors exhibit high rate of heterogeneity estimation of delta- and mu-opioid activities was carried out by means of displacing of 3H-DADLE and 3H-DAGO, respectively. Content of opioid peptides in brain of preadolescent rats was studied during experimental burn trauma using the procedure developed. An increase in the delta- and mu-opioid activities was found in rat brain tissue within one day after thermal burns.
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Reference: Davletov E.G., Kurochkin I.N., Zaĭtsev S.V., Radioreceptor method of determination of opioid peptides in the rat brain, Voprosy meditsinskoi khimii, 1988, vol: 34(4), 136-138.
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