Voprosy meditsinskoi khimii (ISSN 0042-8809)

Use of immunospecific methods for quantitative estimation of microsomal epoxide hydrolase

   
D'iakov V.L., Dubovskoi A.V., Klimova O.A., Sukhanov V.A.
PubMed Id: 2500777
Year: 1989 vol: 35  issue:1  pages: 47-52
Abstract: Antiserum, specific to rat liver microsomal epoxide hydrolase, was produced. Three methods for quantitative estimation of the epoxide hydrolase, base on the principle of antigen-antiserum immunoprecipitation, were used: electroimmune analysis by Laurell, radial immunodiffusion, enzyme-linked immunoassay. Distinct correlation between specific enzyme activity and its content in microsomal preparation was demonstrated using a simple and stable procedure of radial immunodiffusion. In the enzyme-linked assay conjugate of protein A with alkaline phosphate served as the secondary antibody. Sensitivity of the procedure described was about 1,000-fold higher as compared with radial immunodiffusion method.
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Reference: D'iakov V.L., Dubovskoi A.V., Klimova O.A., Sukhanov V.A., Use of immunospecific methods for quantitative estimation of microsomal epoxide hydrolase, Voprosy meditsinskoi khimii, 1989, vol: 35(1), 47-52.
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