Voprosy meditsinskoi khimii (ISSN 0042-8809)

Determination of the amino acid composition of cartilage and creatine phosphokinase isoenzymes with the use of phenylthiocarbamyl amino acid derivatives

   
Romantsev F.E., Prozorovskiĭ V.N., Kozlov E.A., Karpov D.A., Trebukhina E.L., Rzhaninova A.A.
PubMed Id: 2773389
Year: 1989 vol: 35  issue:3  pages: 71-75
Abstract: A method for estimation of amino acid composition and collagen-specific amino acids was developed. The assay was based on the precolumn reaction of amino acids with phenylisothiocyanate. The phenylthiocarbamyl derivatives were analyzed by means of reverse-phase chromatography on octadecyl sorbents, which involved gradient elution with ethanol and sodium or ammonium acetate. The method was used for effective separation of collagen-specific and main amino acids in cartilage hydrolysate from healthy persons and of patients with funnel chest and Ehlers-Danlos syndrome and also for determination of amino acid composition in creatine phosphokinase B- and M-subunits.
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Reference: Romantsev F.E., Prozorovskiĭ V.N., Kozlov E.A., Karpov D.A., Trebukhina E.L., Rzhaninova A.A., Determination of the amino acid composition of cartilage and creatine phosphokinase isoenzymes with the use of phenylthiocarbamyl amino acid derivatives, Voprosy meditsinskoi khimii, 1989, vol: 35(3), 71-75.
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