Voprosy meditsinskoi khimii (ISSN 0042-8809)

Analysis of immunoregulatory peptides of the thymus using high performance liquid chromatography

   
Korotkova M.N., Sanina I.V., Arion V.Ya.
PubMed Id: 2815678
Year: 1989 vol: 35  issue:4  pages: 38-42
Abstract: High performance liquid chromatography (gel filtration and chromatography in the reverse phase) was applied to analyse a composition of the heterogenous immunoregulating peptide preparation T-activine isolated from calf thymus. Gel chromatography at high pressure on the column TSK G2000 SW enabled to separate T-activine into 3 subfractions at a preparative scale. Physico-chemical properties and biological activity of these fractions were studied in vitro. Subfraction B with molecular mass of 1.0-3.5 kD and pI below 5.9 proved to be the most active.
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Reference: Korotkova M.N., Sanina I.V., Arion V.Ya., Analysis of immunoregulatory peptides of the thymus using high performance liquid chromatography, Voprosy meditsinskoi khimii, 1989, vol: 35(4), 38-42.
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