Voprosy meditsinskoi khimii (ISSN 0042-8809)

Quantitative immunoenzyme determination of lactoferrin in the serum and plasma

   
Merkulov A.G., Shevchenko O.P.
PubMed Id: 2697964
Year: 1989 vol: 35  issue:6  pages: 125-128
Abstract: A procedure for quantitative estimation of lactoferrin is developed using the 'sandwich' type of immunoenzymatic assay. Preparation of lactoferrin was purified from women colostrum by means of ion exchange chromatography; the sorbent (a ligand-containing complex of lactoferrin) was synthesized; the antibody conjugate against lactoferrin and peroxidase from horseradish was prepared; optimal conditions of the immunoenzymatic assay were selected. Concentration of lactoferrin in donor blood serum or plasma constituted 0.5-3.3 mg/ml; distinct alterations of the lactoferrin content were not found in blood of patients maintained on artificial circulation or in the blood used for transfusion.
Download PDF:
Reference: Merkulov A.G., Shevchenko O.P., Quantitative immunoenzyme determination of lactoferrin in the serum and plasma, Voprosy meditsinskoi khimii, 1989, vol: 35(6), 125-128.
References
 1995(Vol:41)
 1994(Vol:40)
 1993(Vol:39)
 1992(Vol:38)
 1991(Vol:37)
 1990(Vol:36)
 1989(Vol:35)
 1988(Vol:34)
 1987(Vol:33)
 1986(Vol:32)