Voprosy meditsinskoi khimii (ISSN 0042-8809)

Isolation and purification of collagen proteins using biospecific chromatography on a sorbent with collagen-binding fragment of fibronectin

   
Bychkova V.V., Berman A.E.
PubMed Id: 2629236
Year: 1989 vol: 35  issue:6  pages: 129-133
Abstract: A new procedure is described for isolation of collagens from various preparations containing small amounts of these proteins. The procedure is based on specific affinity of collagens of various types to one of peptide fragments of fibronectin--collagen-binding peptide (CBP). Collagens were isolated after single-step chromatography of tissue homogenate on the sorbent containing immobilized CBP. High specificity and reproducibility of the procedure was shown when various preparations were used: chicken embryo proteins with total radioactive label. Proteins synthesized in fibroblast culture; polypeptides synthesized in cell-free system of protein synthesis.
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Reference: Bychkova V.V., Berman A.E., Isolation and purification of collagen proteins using biospecific chromatography on a sorbent with collagen-binding fragment of fibronectin, Voprosy meditsinskoi khimii, 1989, vol: 35(6), 129-133.
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