Voprosy meditsinskoi khimii (ISSN 0042-8809)

The role of divalent cations in DNA endonucleolysis catalyzed by restrictases

   
Sokolov N.N., Fitsner A.B., Anikeĭcheva N.V., Kovalenko N.A.
PubMed Id: 2629242
Year: 1989 vol: 35  issue:6  pages: 66-69
Abstract: Electrophoretic analysis of products obtained after hydrolysis of phage lambda DNA by means of restrictase Pae I was carried out after preincubation of DNA or the enzyme with Mg2+ as well as after preincubation of DNA simultaneously with the enzyme and the subsequent addition of the required components into the experimental samples. The analysis showed that Mg2+ were apparently not required for the enzyme-substrate complex formation and caused destabilization of the complex. Restrictase Pae I was in active form when Mg2+ was substituted by Mn2+, Ca2+, Zn2+, Co2+ but not by Cd2+, Cu2+ or Ni2+. Experiments with o-phenanthroline showed that Zn2+ cations are of importance in catalytic activity of restrictase Pae I. Possible functions of Zn2+ in protein-nucleic acids recognition are discussed.
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Reference: Sokolov N.N., Fitsner A.B., Anikeĭcheva N.V., Kovalenko N.A., The role of divalent cations in DNA endonucleolysis catalyzed by restrictases, Voprosy meditsinskoi khimii, 1989, vol: 35(6), 66-69.
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