Voprosy meditsinskoi khimii (ISSN 0042-8809)

Antipeptide antibodies specific for the N-terminal site of lactate dehydrogenase from swine muscle

   
Alekseeva A.E., Potapkina T.A., Prozorovskiĭ V.N.
PubMed Id: 2343575
Year: 1990 vol: 36  issue:1  pages: 45-47
Abstract: Antipeptide antisera were obtained after immunization of rabbits with N-terminal peptide of pig muscle lactate dehydrogenase-5 containing the enzyme antigen determinant. The N-terminal peptide (1-32 amino acids) was conjugated with bovine blood serum albumin. Specific antipeptide antibodies were isolated and purified using immunosorbent involving the antigen--lactate dehydrogenase-5. Immunodiffusion procedure and immunoenzymatic assay showed that the antipeptide antibodies obtained were specific towards conjugate, N-terminal peptide and the native pig muscle lactate dehydrogenase-5. The antipeptide antibodies towards N-terminal fragment of lactate dehydrogenase did not affect the enzymatic activity.
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Reference: Alekseeva A.E., Potapkina T.A., Prozorovskiĭ V.N., Antipeptide antibodies specific for the N-terminal site of lactate dehydrogenase from swine muscle, Voprosy meditsinskoi khimii, 1990, vol: 36(1), 45-47.
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