Voprosy meditsinskoi khimii (ISSN 0042-8809)

The effect of antioxidants on the balance of proteinase-inhibitors system in the respiratory space of mice with pulmonary tuberculosis

   
Kaminskaia G.O., Blonskaia G.Yu., Gedymin L.E.
PubMed Id: 2194365
Year: 1990 vol: 36  issue:2  pages: 30-33
Abstract: In three series of experiments generalized form of tuberculosis was simulated in 427 mice of CBA strain. After dissection of chest bronchoalveolar lavage was carried out and in the fluid obtained total content of protein, elastase-like and antitryptic activities were estimated. At the severe stage of the disease content of protein was increased 7-fold, antitryptic activity--5-fold and elastase-like activity--10-fold in the lavage. EDTA did not inhibit the elastase-like activity, i.e. the enzyme was derived only from neutrophils. All the patterns studied were unaltered after the animal treatment with alpha-tocopherol at a dose of 100 mg/kg (per os) within one or three weeks. In vitro incubation of preparations in presence of ascorbic acid at the final concentration of 0.01 M within 1 hr at 37 degrees caused a considerable (4-6-fold) activation of the antitryptic activity simultaneously with a distinct decrease in the elastase-like activity down to physiological values. The data obtained suggest that inactivation of the reactive centre of antiproteinases by means of products of free-radical oxidation appears to be mainly responsible for disbalance of proteinases-inhibitors in tuberculosis.
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Reference: Kaminskaia G.O., Blonskaia G.Yu., Gedymin L.E., The effect of antioxidants on the balance of proteinase-inhibitors system in the respiratory space of mice with pulmonary tuberculosis, Voprosy meditsinskoi khimii, 1990, vol: 36(2), 30-33.
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