Voprosy meditsinskoi khimii (ISSN 0042-8809)

Methodological approaches to evaluation of prostaglandin synthesis by isolated human thrombocytes

   
Geling N.G.
PubMed Id: 2114053
Year: 1990 vol: 36  issue:2  pages: 83-86
Abstract: Individual steps of prostaglandin-synthetase activity evaluation in human platelets are considered in detail, involving isolation of platelets, incubation of washed cells with labelled arachidonic or dihomo-gamma-linolenic acids, extraction of prostaglandins from incubation medium and their separation as well as quantitative estimation of the prostaglandins developed (nmoles/10(9) platelets/10 min). The methodical approaches suggested enabled not only to avoid some errors during estimation of the prostaglandin-synthetase activity in individual platelets but also to increase accuracy, sensitivity and specificity of the procedure as compared with similar methods.
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Reference: Geling N.G., Methodological approaches to evaluation of prostaglandin synthesis by isolated human thrombocytes, Voprosy meditsinskoi khimii, 1990, vol: 36(2), 83-86.
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