Voprosy meditsinskoi khimii (ISSN 0042-8809)

A simple and sensitive method of determination of superoxide dismutase activity based on the reaction of quercetin oxidation

   
Kostiuk V.A., Potapovich A.I., Kovaleva Zh.V.
PubMed Id: 2363268
Year: 1990 vol: 36  issue:2  pages: 88-91
Abstract: An indirect method for determination of superoxide dismutase (SOD) activity, based on inhibition of quercetin autooxidation, was developed. The optimal conditions of reaction ensuring high sensitivity of measurement (I50 = 1.5 ng/ml) were selected. NaN3, catalase and physiological concentrations of ascorbic acid did not interfere with the SOD estimation in blood and tissue homogenates.
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Reference: Kostiuk V.A., Potapovich A.I., Kovaleva Zh.V., A simple and sensitive method of determination of superoxide dismutase activity based on the reaction of quercetin oxidation, Voprosy meditsinskoi khimii, 1990, vol: 36(2), 88-91.
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