Voprosy meditsinskoi khimii (ISSN 0042-8809)

Immunoenzyme method of determination of human alpha2-macroglobulin

   
Kalashnikov V.V., Platonova T.K., Svishchev A.V.
PubMed Id: 1700548
Year: 1990 vol: 36  issue:4  pages: 83-85
Abstract: Highly sensitive, specific immunoenzymatic assay for human alpha 2-macroglobulin was developed using solid phase 'sandwich' procedure. The immunoenzymatic assay developed involved the following steps: 1. preparation of highly purified alpha 2-macroglobulin, 2. production of monospecific antiserum against the protein, 3. preparation of the immunoenzymatic assay system components--isolation of antibodies against alpha 2-macroglobulin, conjugation of these antibodies with horseradish peroxidase, standardization of the assay. Sensitivity of the assay was 31,25 ng/ml, duration of the analysis--3 hrs. The assay enabled to estimate the proteinase inhibitor in various human biological fluids. Content of alpha 2-macroglobulin in blood plasma, urine and tears was estimated in normal and some pathological states.
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Reference: Kalashnikov V.V., Platonova T.K., Svishchev A.V., Immunoenzyme method of determination of human alpha2-macroglobulin, Voprosy meditsinskoi khimii, 1990, vol: 36(4), 83-85.
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