Voprosy meditsinskoi khimii (ISSN 0042-8809)

Express-method of determination of DNA-binding proteins in the mammalian blood

   
Novoradovskaia N.A., Koterov A.N., Filippovich I.V.
PubMed Id: 2238541
Year: 1990 vol: 36  issue:4  pages: 85-88
Abstract: Quick test for estimation of DNA-bound proteins (PBD) in mammalian blood serum involved filtration of blood serum through Wathman filters containing DNA. DNA was fixed in the Wathman filters by means of UV irradiation. Some physicochemical and chromatographic properties of PBD from mice blood serum were studied. The data obtained suggest that blood serum PBD constituted one of gamma-globulin fractions. Applicability of the PBD test for studies of tumoral diseases was shown using mice with Ehrlich ascites carcinoma.
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Reference: Novoradovskaia N.A., Koterov A.N., Filippovich I.V., Express-method of determination of DNA-binding proteins in the mammalian blood, Voprosy meditsinskoi khimii, 1990, vol: 36(4), 85-88.
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