Voprosy meditsinskoi khimii (ISSN 0042-8809)

Increase in the quality of determining estradiol and progesterone receptors in breast tissue cytosol in cancer

   
Khotchenkova N.V., Degtiar' V.G., Bassalyk L.S., Kushlinskii N.E.
PubMed Id: 2075728
Year: 1990 vol: 36  issue:6  pages: 77-79
Abstract: Receptors of estrogens and progestins were studied in cytosol of human mammary gland cancer depending on concentration of total protein. In saturating concentrations of ligand content of these receptors might be distinctly dissimilar when concentration of total protein varied in cytosol, whereas Scatchard plot showed the same phenomena independently on concentration of total protein. Adequate and comparable result in estimation of estrogen and progestin receptors, which is of importance in treatment of mammary gland cancer, may be accomplished by calculation of total protein in cytosol within the limits 1 +/- 0.25 mg ml-1 under saturating concentrations of ligand or using the titrimetric procedure where concentration of total protein constituted 0.7-2.0 mg ml-1.
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Reference: Khotchenkova N.V., Degtiar' V.G., Bassalyk L.S., Kushlinskii N.E., Increase in the quality of determining estradiol and progesterone receptors in breast tissue cytosol in cancer, Voprosy meditsinskoi khimii, 1990, vol: 36(6), 77-79.
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