Voprosy meditsinskoi khimii (ISSN 0042-8809)

Study of oligohemoglobin solutions in vitro and in vivo

   
Kuznetsova N.P., Gudkin L.R., Mishaeva R.N., Stragovich L.M., Samsonov G.V., Selivanov E.A., Bystrova I.M., Gerbut K.A., Molokovskaia I.E., Kochetygov N.I.
PubMed Id: 1907052
Year: 1991 vol: 37  issue:1  pages: 49-51
Abstract: Solutions of oligohemoglobins (OHb) with middle molecular mass 100 x 10(3) = 400 x 10(3), obtained after polycondensation of hemoglobin with glutaric aldehyde as well as oligohemoglobin modified with pyridoxal-5-phosphate, were studied in vivo and in vitro (plethoric administration, isovolemic metabolic substitution, hemorrhagic shock; at a dose of 0.5-1.8 g/kg of body mass; dogs, rabbits, rats). With increase of the OHb molecular mass period of its circulation in blood was elevated; blood plasma protected OHb from autooxidation; half-life of OHb was about 12-18 hrs in isovolemic metabolic substitution and in hemorrhagic shock and about 5 hrs--in plethoric administration. During circulation molecular mass selection of OHb occurred, where low molecular fraction decreased and high molecular fraction--accumulated; sign and value of human erythrocyte charge were similar both in OHb solutions with concentration up to 5% and in physiological solution.
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Reference: Kuznetsova N.P., Gudkin L.R., Mishaeva R.N., Stragovich L.M., Samsonov G.V., Selivanov E.A., Bystrova I.M., Gerbut K.A., Molokovskaia I.E., Kochetygov N.I., Study of oligohemoglobin solutions in vitro and in vivo, Voprosy meditsinskoi khimii, 1991, vol: 37(1), 49-51.
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