Voprosy meditsinskoi khimii (ISSN 0042-8809)

Level of endogenous thiol proteinase inhibitors in subcellular fractions of rat hepatocytes

   
Vasil'ev A.V., Gutkin D.V., Dankova T.G., Vorobeichik T.A., Tutel'ian V.A.
PubMed Id: 1812624
Year: 1991 vol: 37  issue:6  pages: 89-91
Abstract: A procedure for isolation of inhibitors of thiol-dependent proteinases was developed as well as distribution of the inhibitors in rat hepatocyte subcellular fractions was studied. The procedure involved separation of subcellular fractions by means of differential centrifugation, isolation of acid-thermostable proteins and their following purification using affinity chromatography on immobilized papain. The inhibitors were mainly localized in lysosomal, microsomal and cytosol fractions, whereas their specificity towards papain and lysosomal thiol-dependent proteinases cathepsins B, L, H and C was dissimilar, thus suggesting presence of various forms of inhibitors of thiol-dependent proteinases in these cell fractions.
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Reference: Vasil'ev A.V., Gutkin D.V., Dankova T.G., Vorobeichik T.A., Tutel'ian V.A., Level of endogenous thiol proteinase inhibitors in subcellular fractions of rat hepatocytes, Voprosy meditsinskoi khimii, 1991, vol: 37(6), 89-91.
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