Voprosy meditsinskoi khimii (ISSN 0042-8809)

Determination of procollagen type III aminopropeptide by a solid phase method using antibodies conjugated with peroxidase or protein A labelled with radioactive iodine

   
Planutis K.S., Morozevich G.E., Berman A.E.
PubMed Id: 1413636
Year: 1992 vol: 38  issue:2  pages: 57-59
Abstract: A solid-phase assay was developed for estimation of procollagen III aminopropeptide which involved both immunoenzymatic and radioimmune procedures. The assay did not require the iodine-labelled aminopropeptide, while content of the antigen was estimated using the conjugate of secondary antibodies and peroxidase or iodine-labelled protein A. Substitution of the protein A for the antibodies conjugate and peroxidase enabled to abolish the radioactive materials use.
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Reference: Planutis K.S., Morozevich G.E., Berman A.E., Determination of procollagen type III aminopropeptide by a solid phase method using antibodies conjugated with peroxidase or protein A labelled with radioactive iodine, Voprosy meditsinskoi khimii, 1992, vol: 38(2), 57-59.
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