Voprosy meditsinskoi khimii (ISSN 0042-8809)

Physico-chemical, immunochemical, and biological activity of prolactin from human amniotic fluid

   
Bulatov A.A., Makarovskaia E.E., Seganova M.I., Kuznetsov V.P.
PubMed Id: 1413649
Year: 1992 vol: 38  issue:3  pages: 39-43
Abstract: Highly purified preparation of prolactin with molecular mass 23 kDa, which is the main form of the hormone in human amnionic fluid, was isolated from the fluid using gel filtration on Sephadex G-100, chromatography on DEAE-Sepharose, concanavalin A Sepharose, CM-cellulose. Physico-chemical, immunochemical and biological properties of the amnionic prolactin were studied as compared with those of hypophyseal hormone. Properties of the hormonal forms isolated from both these tissues were similar. The glycosylated form of prolactin with molecular mass 25.0 kDa was detected either in amnionic fluid or in hypophysis.
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Reference: Bulatov A.A., Makarovskaia E.E., Seganova M.I., Kuznetsov V.P., Physico-chemical, immunochemical, and biological activity of prolactin from human amniotic fluid, Voprosy meditsinskoi khimii, 1992, vol: 38(3), 39-43.
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