Voprosy meditsinskoi khimii (ISSN 0042-8809)

Use of monoclonal antibodies for immunosorption of growth hormone from human biological tissues and fluids

   
Bulatov A.A., Osipova T.A., Grigor'ian A.L., Fedotov V.P.
PubMed Id: 8122403
Year: 1994 vol: 40  issue:1  pages: 21-25
Abstract: An immunosorbent with high binding capacity was developed using monoclonal antibodies specific to human growth hormone. Potential activity of the sorbent was studied in isolation of growth hormone from biological tissues and fluids. The immunosorbent enabled one to isolate the biologically and immunologically active growth hormone with high purity from the hypophyseal extract using only one step of purification. As shown by affinity chromatography and subsequent electrophoresis in polyacrylamide gel combined with radioimmunoblotting, together with predominant form of growth hormone, also intact and secreted from hypophysis, two-chain form of the hormone arising after proteolysis and large immunoreactive hormone fragments were detected in human circulation. These derivatives of the hormone may be also excreted with urine in unaltered form.
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Reference: Bulatov A.A., Osipova T.A., Grigor'ian A.L., Fedotov V.P., Use of monoclonal antibodies for immunosorption of growth hormone from human biological tissues and fluids, Voprosy meditsinskoi khimii, 1994, vol: 40(1), 21-25.
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