Voprosy meditsinskoi khimii (ISSN 0042-8809)

Optimization of determining human erythrocyte glutathione reductase activity using a semiautomatic analyzer

   
Mal'tsev G.Iu., Orlova L.A.
PubMed Id: 8160436
Year: 1994 vol: 40  issue:2  pages: 59-61
Abstract: A relatively simple procedure was developed for estimation of the glutathione reductase activity using half-automatic analyzer. The procedure involved: preparation of hemolysates with minimal rate of erythrocyte destruction; substitution of buffers with high ionic strength for 0.05 M Tris-HCl buffer not containing EDTA. Most effective period of preincubation was found; dependence of the reaction rate on FAD was studied. The procedure developed was approved using two types of half-automatic analyzer and the model FP-901 was shown to be the most suitable for estimation of the glutathione reductase holoenzyme activity.
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Reference: Mal'tsev G.Iu., Orlova L.A., Optimization of determining human erythrocyte glutathione reductase activity using a semiautomatic analyzer, Voprosy meditsinskoi khimii, 1994, vol: 40(2), 59-61.
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