Voprosy meditsinskoi khimii (ISSN 0042-8809)

A comparative study of aspartyl and cysteine proteinases and their inhibitors in human B- and T-cell leukemias

   
Gureeva T.A., Golubeva N.V., Lubkova O.N., Lokshina L.A.
PubMed Id: 8079445
Year: 1994 vol: 40  issue:3  pages: 6-8
Abstract: A procedure was developed for simultaneous isolation of aspartyl and cysteine proteinases as well as of the cysteine-proteinase inhibitors. Affinity chromatography using pepstatin-Sepharose enabled one to isolate aspartyl proteinases, while inhibitors of cysteine-proteinases were isolated by affinity chromatography on CM-papain-Sepharose; further purification of the enzymes was carried out using ion exchange chromatography and gel filtration. Partially purified preparations of cathepsin D as well as of cysteine-proteinases and their inhibitors were obtained. Some physicochemical and enzymatic properties of the enzymes and inhibitors obtained were studied.
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Reference: Gureeva T.A., Golubeva N.V., Lubkova O.N., Lokshina L.A., A comparative study of aspartyl and cysteine proteinases and their inhibitors in human B- and T-cell leukemias, Voprosy meditsinskoi khimii, 1994, vol: 40(3), 6-8.
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