Voprosy meditsinskoi khimii (ISSN 0042-8809)

Determination of metabolism of steroid hormones and activity of their synthetic enzymes in murine Leydig cells by high performance liquid chromatography

   
Svechnikov K.V., Busygina T.V., Osadchuk A.V.
PubMed Id: 7975370
Year: 1994 vol: 40  issue:4  pages: 14-17
Abstract: A very effective procedure was developed for separation of sex hormones of the delta 4 pathway using the microcolumn technique of high performance liquid chromatography (HPLC). A five-step gradient containing methanol, acetonitrile and water was used as a mobile phase, while Lychrosorb RP-18 with particles of 5 microns was used as a sorbent. Metabolism of steroid hormones and activity of 3 beta-steroid dehydrogenase, 17 alpha-hydroxylase, C17-20-lyase and 17-ketosteroid reductase were studied in Leydig cells of two mouse strains PT and A/He. The higher rate of steroid metabolism and of the enzymatic activity was detected in the mouse cells of PT strain as compared with those of A/He strain.
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Reference: Svechnikov K.V., Busygina T.V., Osadchuk A.V., Determination of metabolism of steroid hormones and activity of their synthetic enzymes in murine Leydig cells by high performance liquid chromatography, Voprosy meditsinskoi khimii, 1994, vol: 40(4), 14-17.
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