Voprosy meditsinskoi khimii (ISSN 0042-8809)

Isolation of lactate dehydrogenase-C4 from human spermatozoa

Sevriukov E.A., Evseev L.P.
PubMed Id: 7839663
Year: 1994 vol: 40  issue:5  pages: 23-25
Abstract: Lactate dehydrogenase-C4 (LDH) is a specific cell isoenzyme of spermatogenesis and spermatozoa of man and animals. Due to the lower levels of contaminating proteins, isolation of LDH C4 from spermatozoa had a lot of advantages. LDH C4 was isolated as follows: precipitation with ammonium sulfate, separation of LDH C4 from other LDH isoenzymes using immunosorbents, purification from contaminating proteins of oxamate-Sepharose. Antibodies to LDH1-3 immobilized on tresyl-Sepharose were used as immunosorbents. Elution of LDH C4 from oxamate-Sepharose by means of lithium lactate appeared inadequate therefore LDH C4 was eluted using 0.63 mM sodium pyruvate.
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Reference: Sevriukov E.A., Evseev L.P., Isolation of lactate dehydrogenase-C4 from human spermatozoa, Voprosy meditsinskoi khimii, 1994, vol: 40(5), 23-25.