Voprosy meditsinskoi khimii (ISSN 0042-8809)

Oxidative modification of human serum proteins. A method of determining it

   
Dubinina E.E., Burmistrov S.O., Khodov D.A., Porotov I.G.
PubMed Id: 7771084
Year: 1995 vol: 41  issue:1  pages: 24-26
Abstract: A rate of protein oxidative destruction may be estimated from the reaction of the resultant carbonyl derivatives of amino acids reaction with 2,4-dinitrophenyl hydrazine. The procedure for estimation of 2,4-dinitrophenyl hydrazones was modified for clinical application. The initiating effect of nonenzymatic components (Fe(3+)-ascorbic acid, Fe(2+)-O2, Fe(2+)-H2O2) was in the metal-catalyzing oxidation of proteins. Estimation of derivatives of 2,4-dinitrophenyl hydrazones in tissues may serve as a pattern of protein oxidative modification during oxidative stress in the body
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Reference: Dubinina E.E., Burmistrov S.O., Khodov D.A., Porotov I.G., Oxidative modification of human serum proteins. A method of determining it, Voprosy meditsinskoi khimii, 1995, vol: 41(1), 24-26.
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