Voprosy meditsinskoi khimii (ISSN 0042-8809)

Rapid accumulation of ATP in enriched cell membrane particle substances made from bovine aortic endothelial cells and human hemangioma under the influence of endothelial growth factor

   
Karelin A.A., Globa A.G., Vtiurin B.V., Pisarzhevskiĭ S.A., Demidova V.S., Dan V.N.
PubMed Id: 8585169
Year: 1995 vol: 41  issue:3  pages: 17-20
Abstract: Endothelial cell growth factor (ECGF) is a peptide synthesized in the hypothalamus and platelets, which regulates a process of angiogenesis, stimulating the vascular endothelial cell proliferation of and formation of new capillaries. As previously shown that activation of target cells in response to insulin, growth factors, stimulators of mitogenesis and chemotaxis, followed by phosphorylation of membrane components, occurs via generation of 'signal' ATP on plasma membranes of these target cells. ATP was synthesized within 1 min in the preparations enriched with plasma membrane particles obtained from the endothelium of the bovine aorta and human hemangioma and washed in 0.25 M sucrose, the incubated in the medium containing ECGF 150 micrograms/ml, Tris-HCl pH 7.5, ADP, Mg2+, inorganic P during NADH oxidation in the presence of cytochrome c and oxygen. The stimulation of ECGF on ATP formation in the plasma membranes was detected in 9 experiments when kinases inhibitor of 5-fluorosulfonyl benzoyladenosine was used. The ECGF-stimulated ATP production was equal to 6.1-27.4 and 21.6 nmol/min/mg protein in the membranes of the bovine aortic endothelium and human hemangioma, respectively at 30 degrees. Plasma membrane signal ATP appears to serve as a secondary 'membrane messenger' for ECGF.
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Reference: Karelin A.A., Globa A.G., Vtiurin B.V., Pisarzhevskiĭ S.A., Demidova V.S., Dan V.N., Rapid accumulation of ATP in enriched cell membrane particle substances made from bovine aortic endothelial cells and human hemangioma under the influence of endothelial growth factor, Voprosy meditsinskoi khimii, 1995, vol: 41(3), 17-20.
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