Voprosy meditsinskoi khimii (ISSN 0042-8809)

Use of a nylon membrane for purifying DNA fragments from agarose gels

   
Kulikov A.V.
PubMed Id: 8585186
Year: 1995 vol: 41  issue:3  pages: 61-62
Abstract: A new and effective technique was proposed for rapid 30-40 minisolation of pure double-stranded DNA fragments from agarose gels and their use for nick-translation, for restriction with cutting enzymes and ligation with vector DNA. The technique developed involved an electrophoretic transferring of a DNA fragment from the gel into a strip of nylon membrane with the pore size of 0.2 mm followed by elution with buffer containing 0.1% SDS. Yield of the DNA fragment with a length up to 8.0 Kbp was at least 50%. Advantages of the technique developed as compared with standard methods are discussed
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Reference: Kulikov A.V., Use of a nylon membrane for purifying DNA fragments from agarose gels, Voprosy meditsinskoi khimii, 1995, vol: 41(3), 61-62.
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