Voprosy meditsinskoi khimii (ISSN 0042-8809)

Enzyme immunoassay for staphylococcal toxin with the use of polyamide micropore membranes

   
Tashmukhamedova Sh.S., Rakhimov M.M., Sultanov Kh.K.
Type: Experimental study
PubMed Id: 8999657
Year: 1996 vol: 42  issue:1  pages: 39-45
Abstract: The optimum conditions for extraction of catalytic active conjugates of alpha-amylase and antibodies for staphylococcal toxin (ST) have been created. The optimum correlation of antibodies and enzyme for the effective use of the extracted conjugate during enzyme immunoassay for ST has been established. The covalent immobilization of antibodies on the micropore polyamide membranes has been carried out. Apart from this, the extracted conjugated were purified by TSK-gel HN-55 chromatography. The optimum concentration of the conjugates has been defined.
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Reference: Tashmukhamedova Sh.S., Rakhimov M.M., Sultanov Kh.K., Enzyme immunoassay for staphylococcal toxin with the use of polyamide micropore membranes, Voprosy meditsinskoi khimii, 1996, vol: 42(1), 39-45.
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