Voprosy meditsinskoi khimii (ISSN 0042-8809)

Some peculiarities of the liver tissue fructose 2,6-bisphosphate system in experimental streptozocin diabetes

   
Markova M.S., Golubev M.A., Gorodetsky V.K., Belyaeva N.F., Viktorova L.N., Korovkin B.F.
Type: Experimental study
PubMed Id: 9139454
Year: 1996 vol: 42  issue:3  pages: 223-226
Abstract: The activity of phosphofructokinase-2, fructose, 1,6-bisphosphatase, glucokinase, and also the level of fructose 2,6-bisphosphate and glycogen were examined in the liver of normal, and streptozotocin-diabetic rats. It was shown that the activity of phosphofructokinase-2 was decreased in the liver of diabetic rats. Besides that the activity determined at pH 6.6 (the 'active' or unphosphorylated enzyme form) was 3-fold reduced whereas the 'total' enzyme activity as measured at pH 8.5 was lowered 1,7-fold. The phosphofructokinase-2 activity assay at two pH values allows to estimate a degree of phosphorylation of bifunctional enzyme which is markedly enhanced in diabetes. The fall of the bifunctional enzyme k in case activity is accompanied by the lowered fructose 2.6-bisphosphate level, increased fructose 1,6-bisphosphatase activity that in turn favours the liver tissue glycolysis inhibition and gluconeogenesis enhanced in diabetes.
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Reference: Markova M.S., Golubev M.A., Gorodetsky V.K., Belyaeva N.F., Viktorova L.N., Korovkin B.F., Some peculiarities of the liver tissue fructose 2,6-bisphosphate system in experimental streptozocin diabetes, Voprosy meditsinskoi khimii, 1996, vol: 42(3), 223-226.
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