Voprosy meditsinskoi khimii (ISSN 0042-8809)

Gene-engineering approaches to study the markers for insulin dependent diabetes mellitus

   
Pankov A.Yu.
PubMed Id: 9446325
Year: 1997 vol: 43  issue:5  pages: 349-355
Abstract: In the present study, gene-engineering producers for proinsulin and peptidefragments of glutamic acid decarboxylase (GAD), the main autoantigens ofislet cells responsible for development of insulindependent diabetes mellitus(IDDM), were prepared. Basing on the obtained antigens, ELISA was developedto determine the autoantibodies in sera of patients. Sera from the followingtwo groups of IDDM patients were studied: 1) adult patients with recentlydeveloped IDDM before insulinotherapy, and 2) adult patients with long-lastingIDDM with associated complications. Insulin/proinsulin antibodies weredetected in 20% patients of group 1 and 41.7% patients of group 2. In serafrom IDDM patients, antibodies to two GAD fragments containing amino acidsequences 1-80 and 151-585 were also detected. 16% of sera from patientswith recently developed IDDM and 37.5% of sera from patients with long-lastingIDDM reacted with N-terminal GAD fragment. 12% of sera from group 1 and45.8% of sera from group 2 reacted with central-C-terminal GAD fragment.The rate of sera of IDDM patients with detected antibodies in this studywas some lower than that described in the literature.
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Reference: Pankov A.Yu., Gene-engineering approaches to study the markers for insulin dependent diabetes mellitus, Voprosy meditsinskoi khimii, 1997, vol: 43(5), 349-355.
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