Abstract: Interaction of transcription factors with functional regions ofgene mdr1 promoter has been studied. Binding of transcriptionfactors to double-stranded (ds) oligonucleotides, mimicking fourimportant regulatory regions before and after treatment of KB andK-562 cell lines with doxorubicin, cytarabine, and vinblastinewas analysed by gel-shift assay. Gene induction resulteds inenhancement of the main complex A(A1) proteins formation on theall four regions in both the cell lines. Inhibition of eachcomplex formation by an excess of non-radiolabelled dsoligonucleotides suggests the possibility of regions interactionduring promoter activation. Another indication of interaction ofregulatory regions during activation is overlapping minorcomplexes sets. The results suggest that the four studied regulatory regions areimportant for mdr1 gene activation. Correspondingoligonucleotides mimicking these regions can be employed asinhibitors of gene transcription that bind specifictranscriptional factors.
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Reference: Bozhenok L.N., Kolpakova A.V., Vladimirova A.V., Chernolovskaya E.L., Vlasov V.V., Investigation of transcription factors binding to functional regions of the gene MDR1 promoter during its activation, Voprosy meditsinskoi khimii, 1999, vol: 45(1), 37-46.
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