Voprosy meditsinskoi khimii (ISSN 0042-8809)

A new approach to the investigation of adrenaline autooxidation and its application for determination of superoxide dismutase activity

   
Sirota T.V.
PubMed Id: 10432564
Year: 1999 vol: 45  issue:3  pages: 263-272
Abstract: The intensive rise of absorptionh maximum at 347 nm (wavenumber 28.8) was found during autoxidation of low concentrations of adrenaline (230 m M) in alkaline pH (10,65) under room temperature and in the absenceof additional sources of superoxide. It was stated that the appearance of this product ofadrenaline oxidation, which was not reported earlier, is considerably more rapid thanformation of adrenochrome (absorbtion at 480 nm) and is inhibited by superoxide dismutase(SOD). It was shown that erythrocyte hemolysate and some studied antioxidants (ascorbateand cysteine) inhibit formation of this substanse like SOD. The determination of thedescribed substance can be used for measurement of both: activity of SOD or antioxidantactivity of different biological materials. The possibility to use available not expensivechemicals (0.1% adrenaline solution from drug store, 0.2 M bicarbonate buffer) serves asan additional advantage of the proposed method.
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Reference: Sirota T.V., A new approach to the investigation of adrenaline autooxidation and its application for determination of superoxide dismutase activity, Voprosy meditsinskoi khimii, 1999, vol: 45(3), 263-272.
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