Voprosy meditsinskoi khimii (ISSN 0042-8809)

Affinity chromatograpy of DNA-protein complexes

   


1. St.Petersburg Institute of Bioregulation and Gerontology, North-Western Department of the Russian Academy of Medical Sciences
2. Institute of Macromolecular Compounds, Russian Academy of Sciences
PubMed Id: 10802888
Year: 2000 vol: 46  issue:1  pages: 62-71
Abstract: Affinity sorbentcontaining cellular membranes of the cerebral cortex was obtained by means of an inclusioninto porous matrix of polyacrilonitril. Sorption of model and natural nucleoproteincomplexes on the obtained sorbent was studied according to the method of affinitychromatography. Distribution coefficients were defined. As it was revealed, the naturalnucleoprotein complexes isolated from brain possesses a greater selectivity of bindingwith the cellular membranes of the cerebral cortex in comparison with that from the liver.
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Reference: Riadnova I.Yu., Chernova I.A., Shataeva L.K., Khavinson V.Kh., Affinity chromatograpy of DNA-protein complexes, Voprosy meditsinskoi khimii, 2000, vol: 46(1), 62-71.
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