Voprosy meditsinskoi khimii (ISSN 0042-8809)

The level of blood serum 7a -hydroxycholesterol as an indicator of cholesterol catabolism under hyperlipidemia and hypolipidemic therapy

   


1. Institute of Biomedical Chemistry RAMS
2. National Research Centre for Preventive Medicine
PubMed Id: 10885038
Year: 2000 vol: 46  issue:2  pages: 168-175
Abstract: The effect of micronised fenofibrate (Lipanthyl 200M, Laboratoire Fournier, France, in dose 200 mg per day) on the serum level of 7a -hydroxycholesterol (7a -(OH)C) was studied in 10 men (aged 31-60 years) with hyperlipidemia (total C> 6.5 mmol/l). The levels of 7a -(OH)C as well as that of total C, LDL C, VLDL C and HDL C and triglycerides (Tg) were measured before Lipanthyl 200 M treatment (point 0) and after 1, 2 and 3 months of the drug administration. The content of 7a -(OH)C was determined by the reversed phase HPLC after the enzymatic conversion of serum 7a -(OH)C to 7a -(OH)-4-cholesten-3-one in cholesterol oxidase reaction. 7b -(OH)C was used as the internal recovery standard. Lipanthyl treatment resulted in considerable reduction of total C, VLDL C and LDL C levels and 7a -(OH)C content. Affor the second and third months of therapy serum levels of 7a -(OH)C were significantly reduced from 1.3± 0.1 to 0.8± 0.2 and 0.7± 0.1 m mol/l; (P<0.02). The decrease of 7a -(OH)C content was associated with the decrease in Tg and VLDL C levels. Thus, our data suggest that the level of 7a -(OH)C of in human serum may be used as an indicator of intensity of cholesterol oxidation into bile acids.
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Reference: Polyakova E. D., Ivanova T. N., Voschinnikov E. I., Olferiev A. M., Perova N.V., The level of blood serum 7a -hydroxycholesterol as an indicator of cholesterol catabolism under hyperlipidemia and hypolipidemic therapy, Voprosy meditsinskoi khimii, 2000, vol: 46(2), 168-175.
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