Voprosy meditsinskoi khimii (ISSN 0042-8809)

Non-viral genedelivery in vivo for gene therapy


1. Research Institute for Biomedical Chemistry, RAMS
PubMed Id: 11033883
Year: 2000 vol: 46  issue:3  pages: 226-245
Abstract: Recent findings connected with in vivo use of artificialmacromolecular complexes (genosomes) for functional gene transfer and delivery arediscussed in the paper. Non-viral methods are the most safe for the purpose of human genedelivery. The cationic liposomes containing cholesterol are the most suitable for thispurpose, because they possess high biodegradability and stability in blood stream. TheDNA-liposome complexes should: (i) contain DNA in the condition at most protected fromenviromental influence, (ii) be rather homogeneous and of small size (40-80 nm).Injections of complexes into blood are the most effective; in a respect of organospecifitymay be achieved by appropriate ligand selection. It is the most perspectively to increasethe expression level by combining liposomes with viral peptides.
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Reference: Bogdanenko E.V., Sviridov Yu.V., Moskovtsev A.A., Zhdanov R.I., Non-viral genedelivery in vivo for gene therapy, Voprosy meditsinskoi khimii, 2000, vol: 46(3), 226-245.