Voprosy meditsinskoi khimii (ISSN 0042-8809)

Photochemiluminescence as a tool to determine the antioxidant activity in biological systems. Mathematic modeling

   


1. RESEARCH INSTITUTE FOR ANTIOXIDANT THERAPY CO. (F.A.T. CO)
2. MOSCOW STATE UNIVERSITY, FACULTY OF BASIC MEDICINE, DEPARTMENT OF PHYSICO-CHEMICAL BASICS OF MEDICINE
PubMed Id: 11075425
Year: 2000 vol: 46  issue:4  pages: 419-426
Abstract: The mathematical modeling of the kinetics of riboflavin photochemiluminescence (PCL) in the presence of antioxidants, superoxide dismutase and ascorbic acid, has been performed. A specially developed computer program “Kinetic Analyzer” was used for the modeling. The PCL intensity of was taken as directly proportional to the superoxide concentration, because lucigenin had been added to the system. It was found, that the experimental curves of PCL virtually coincide with those calculated in the case of the following set of reactions (reaction rate constants, M-1·s–1 are given in brackets): hn + RH ® R· + ·O2– (2,3·10–4 s–1); RH + ·O2– ® R· + H2O2 (1000); RH ® … (0,005 s–1); ·O2– + ·O2– ® … + фотон (2·105 М–1 s–1); SOD + ·O2– ® SOD + H2O2 (1·108); ASC + ·O2–® …(2·107). Here RH is , ·O2– – superoxide , R· – riboflavin radical, SOD – superoxide dismutase, ASC – ascorbate
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Reference: Lewin G., Magin D.V., Ismailov D.Yu., Popov I.N., Vladimirov Yu.A., Photochemiluminescence as a tool to determine the antioxidant activity in biological systems. Mathematic modeling, Voprosy meditsinskoi khimii, 2000, vol: 46(4), 419-426.
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