Voprosy meditsinskoi khimii (ISSN 0042-8809)

Gamma-plant, modifies in vitro release of TNF-?, IL-1? and IL-6by human peripheral blood mononuclear cells


1. The Institute of Molecular Genetics of Russian Academy of Sciences
2. Research Institute for Microbiology and Epidemiology of Gabrichevskogo G.N Ministry of Public Health
PubMed Id: 11450448
Year: 2001 vol: 47  issue:2  pages: 263-255
Abstract: The effect of a new medicine gamma-plant hasantiinflammatory activity on human peripheral blood mononuclear cells obtained fromhealthy donors was studied. Its ability to influence TNF, IL-1 and IL-6 production viamononuclear cells was determined. Lymphokines spontaneous production and lymphokinesproductions by cells stimulated by LPS was studied. IL-1 content was defined withIL-1sensitive cell line D10G4.1 while IL-6 content with IL-6 dependent heterohybridomaD6C8. TNF activity in supernatants was determined as lysis grade ofTNF-sensitive cells ofmouse fibrosarcoma L-929. It was shown that gamma-plant acted as stimulator when IL-1production was low and as inhibitor when it was high. Stimulation of IL-6 productioninduced with LPS was achieved by low gamma-plant doses while for stimulation ofspontaneous production higher doses were required. After nonstimulated LPS peripheralblood cells were treated with gamma-plant statistically sensible stimulated TNF productionwas observed apart from TNF synthesis level in a control group. The TNF productioninhibition could never be demonstrated in subjects that showed in control culturesstimulated with LPS a level of TNF production lower than 100 pg/ml. In four of the fivehigh TNF producers the cytokine release inhibition in LPS stimulated cultures has beenobserved. It is likely that the mild stimulation of the proinflammatory cytokine systemhas taken place. For example, simultaneously with stimulation of cytokine production thecirculating IL-1 receptor antagonist and/or soluble TNF receptor may be induced.
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Reference: Chekanovskaya L.A., Generalov A.V., Gamma-plant, modifies in vitro release of TNF-?, IL-1? and IL-6by human peripheral blood mononuclear cells, Voprosy meditsinskoi khimii, 2001, vol: 47(2), 263-255.